mouse anti-fetuin antibody  (Millipore)

Journal: Journal of pharmacy and pharmacology research

Article Title: Fetuin-A Modulates Tumor Growth and Invasion in a Basal-like Triple Negative Breast Cancer Cell line, MDA-MB-468

doi: 10.26502/fjppr.0103

Figure Lengend Snippet: Overexpression of fetuin-A (Fet-A) in MDA-MB-468 TNBC cell line. MDA-MB-468 cells were transfected with either empty vector (EV) or flag-tagged fetuin-A expression vector (FA). Fetuin-A expression level was determined by QT-RTPCR (panel A), Western blot (panel B) and IHC (panel C) as described in .

Article Snippet: Primary mouse monoclonal anti-human fetuin-A/AHSG antibody (R&D Systems, MAB1184) was used at a dilution of 1:500 in Antibody OP Quanto antibody diluent (Thermo Fisher, TA-125-ADQ).

Techniques: Over Expression, Transfection, Plasmid Preparation, Expressing, Reverse Transcription Polymerase Chain Reaction, Western Blot

Journal: Journal of pharmacy and pharmacology research

Article Title: Fetuin-A Modulates Tumor Growth and Invasion in a Basal-like Triple Negative Breast Cancer Cell line, MDA-MB-468

doi: 10.26502/fjppr.0103

Figure Lengend Snippet: Enhanced invasion capacity in fetuin-A overexpressing MDA—MB-468 cells. The MDA-MB-468-FA invaded through a bed of Matrigel more readily compared to MDA-MB-468-EV when complete medium (CM) was in the lower compartment of the trans-well (*P<0.05); N=6). Both sub-clones did not invade when SFM was in the lower compartments (controls) (**P<0.001; N=6)

Article Snippet: Primary mouse monoclonal anti-human fetuin-A/AHSG antibody (R&D Systems, MAB1184) was used at a dilution of 1:500 in Antibody OP Quanto antibody diluent (Thermo Fisher, TA-125-ADQ).

Techniques: Clone Assay

Journal: Journal of pharmacy and pharmacology research

Article Title: Fetuin-A Modulates Tumor Growth and Invasion in a Basal-like Triple Negative Breast Cancer Cell line, MDA-MB-468

doi: 10.26502/fjppr.0103

Figure Lengend Snippet: Toll like receptor 4 (TLR4) expression in MDA-MB-468 sub-clones. Surface TLR4 expression levels were determined by flow cytometry in MDA-MB-468-EV (upper panels) and MDA-MB-468-FA (lower panels) in the absence and presence of added fetuin-A (FA)

Article Snippet: Primary mouse monoclonal anti-human fetuin-A/AHSG antibody (R&D Systems, MAB1184) was used at a dilution of 1:500 in Antibody OP Quanto antibody diluent (Thermo Fisher, TA-125-ADQ).

Techniques: Expressing, Clone Assay, Flow Cytometry

Journal: Journal of pharmacy and pharmacology research

Article Title: Fetuin-A Modulates Tumor Growth and Invasion in a Basal-like Triple Negative Breast Cancer Cell line, MDA-MB-468

doi: 10.26502/fjppr.0103

Figure Lengend Snippet: Fetuin-A mediates 2-D and 3-D growth of MDA-MB-468 via TLR4 signaling. The cells were seeded (2 × 10 4 cells/well) in attachment (2-D) ( panel A ) or ultra-low attachment (3-D) ( panel B ) 96-well plates in SFM; SFM + CLI-095 (CLI); Fetuin-A (FA); FA + CLI; complete medium (CM); and in CM + CLI. After 6 days of growth, Alamar blue was added to each well, incubated for 2 h and fluorescence measured as described. *P< 0.05; **P< 0.001; ***P<0.0001; N= 4

Article Snippet: Primary mouse monoclonal anti-human fetuin-A/AHSG antibody (R&D Systems, MAB1184) was used at a dilution of 1:500 in Antibody OP Quanto antibody diluent (Thermo Fisher, TA-125-ADQ).

Techniques: Incubation, Fluorescence

Journal: Journal of pharmacy and pharmacology research

Article Title: Fetuin-A Modulates Tumor Growth and Invasion in a Basal-like Triple Negative Breast Cancer Cell line, MDA-MB-468

doi: 10.26502/fjppr.0103

Figure Lengend Snippet: In A , control chambers had only cells in SFM, while experimental chambers had CLI-095 (28 µM) in SFM (upper chambers) of trans-well assay. The bottom chambers had complete medium (CM). In B , controls had CM only in the bottom chambers while experimental chambers had CM depleted of fetuin-A (CM + HA). Number of invading cells was determined as described in .

Article Snippet: Primary mouse monoclonal anti-human fetuin-A/AHSG antibody (R&D Systems, MAB1184) was used at a dilution of 1:500 in Antibody OP Quanto antibody diluent (Thermo Fisher, TA-125-ADQ).

Techniques: Control